pCDNA3.1(+)哺乳表达质粒

基本信息

质粒属性

质粒简介

pCDNA3.1(+)是来自pCDNA3的5.4kb载体，设计用于哺乳动物宿主中的高水平稳定和瞬时表达。大多数哺乳动物细胞可以进行高水平的稳定和非复制性瞬时表达。人类巨细胞病毒立即早期（CMV）启动子，用于在广泛哺乳动物细胞中的高水平表达。前向（+）和反向（-）方向上的多个克隆位点，以促进克隆用于选择稳定细胞系的新霉素抗性基因。在潜伏感染SV40或表达SV40大T抗原（例如COS-1，COS-7）的细胞系中的异常复制。

pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

• Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells

• Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning

• Neomycin resistance gene for selection of stable cell lines

• Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.

This pcDNA3.1(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.

pCDNA3.1(+)的参考文献：Clinical significance of bromodomaincontaining protein 7 and its association with tumor progression in prostate cancer.

质粒图谱

质粒序列

质粒序列请下载：

pCDNA3.1(+)哺乳表达质粒.txt