SM10λpir感受态细胞

产品介绍

Donor strain (mobilizing strain) carrying the transfer genes of the broad host range IncP type plasmid RP4 integrated in its chromosome.Useful for mobilizing mobRP4 plasmids.The presence of λpir allows the replication of a defective R6K suicide plasmid and derivatives thereo.E. coli SM10 λpir strain contains the pir gene (lysogenized with λpir phage) and it is designed for cloning and propagation of plasmids with R6K origin of replication sequence (such as pUT mini-Tn5 vectors). The plasmids containing the R6K origin of replication require the R6K-spicific replication protein lpir gene for propagation.

SM10λpir感受态细胞主要用于结合转移实验。SM10λpir感受态细胞经特殊工艺制作，pUC19质粒检测转化效率>10⁶cfu/μg DNA。

基因型

thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu

说明书下载

YC141 SM10λpir感受态细胞.pdf